Bioradtopgm Command (with examples)

1. Convert a Biorad confocal file into a PGM file

bioradtopgm -n path/to/file.pic > path/to/file.pgm

Motivation: This command is used to convert a Biorad confocal file (in .pic format) into a Portable Gray Map (PGM) file. PGM is a simple and portable image format commonly used in computer vision and image processing applications. By converting the Biorad confocal file to PGM, it allows us to work with the image data more easily and perform various image processing tasks.

Explanation:

• -n: Specifies the index of the image within the Biorad confocal file that needs to be converted. Replace n with the desired image index, starting from 0.
• path/to/file.pic: Specifies the path to the Biorad confocal file that needs to be converted.
• path/to/file.pgm: Specifies the path where the converted PGM file will be stored.

Example Output: The n’th image from the Biorad confocal file will be converted to a PGM file and saved at the specified path (path/to/file.pgm).

2. Read a Biorad confocal file and print the number of images it contains

bioradtopgm path/to/file.pic

Motivation: This command is used to determine the number of images stored within a Biorad confocal file. It can be useful to know the total number of images in order to perform further analysis or to determine the size of the dataset contained in the file.

Explanation:

• path/to/file.pic: Specifies the path to the Biorad confocal file for which you want to determine the number of images.

Example Output: The command will read the Biorad confocal file specified and print the number of images it contains.

3. Display version

bioradtopgm -version

Motivation: This command allows you to check the version of the bioradtopgm command-line tool installed on your system. It is useful for verifying the tool’s compatibility or determining if any updates are available.

Explanation: No additional arguments are required for this command.

Example Output: The command will display the version number of the bioradtopgm tool.